Evaluation of Diabetic Wound Healing Activity by Topical Application of Gallic Acid Loaded Chitosan Composite Scaffold on Streptozotocin-Induced Diabetic Rats

Abstract

Diabetic foot ulcers are complicated sores that interfere with the skin's functional activity and, more critically, the essential role of skin cells. Gallic acid is a plant-derived polyphenol-rich substance that has been linked to health benefits such as antioxidant and anti-inflammatory action. The research will focus on generating and improving gallic acid-loaded chitosan-gelatin composite scaffolds for diabetic wound healing. The scaffolds are then evaluated for their physicochemical characteristics, cell viability is determined using the in-vitro cytotoxicity technique, and putative wound healing activity in full-thickness excision wounds in an in-vivo model of streptozotocin-induced diabetic rats is investigated. The freeze-drying process was utilized to prepare the formulation (Scaffolds). Porosity, water absorption, and matrix degradation Preformulation experiments were carried out (69.8%, 32.07%). The in-vitro drug release research indicated that 52.55% of the medication is released after 48 hours, while the in-vitro cytotoxicity study found that the IC50 was 32.1g/mL. in-vivo studies have been conducted for Dermal irritation. By injecting STZ 60mg/dL in a group of rats and establishing diabetic wounds, researchers were able to examine wound contraction, hexosamine content, and hydroxyproline content. The studies were completed and reported on.